Specific binding of perhydrohistrionicotoxin to Torpedo acetylcholine receptor.
نویسندگان
چکیده
منابع مشابه
Long-lived metastable states and hysteresis in the binding of acetylcholine to Torpedo california acetylcholine receptor.
Studies of the binding of [3H]acetylcholine to receptor-rich membranes of Torpedo californica electric organ under conditions that normally lead to a state of equilibrium did not give rise to equilibrium binding curves. Instead, the acetylcholine receptor was found to develop very long lived metastable states resulting in hysteresis in binding. Under conditions where the concentration of free [...
متن کاملBinding of perhydrohistrionicotoxin to intact and detergent-solubilized membranes enriched in nicotinic acetylcholine receptor.
A tritiated derivative of perhydrohistrionicotoxin was prepared and characterized and used in direct binding studies to membrane fragments from Torpedo californica electroplax. Perhydrohistrionicotoxin bound to a site distinct from the a-bungarotoxin and agonist binding sites in a ratio of one perhydrohistrionicotoxin bound per four a-bungarotoxin sites. In the presence of 10 pM carbamylcholine...
متن کاملActivation, inactivation, and desensitization of acetylcholine receptor channel complex detected by binding of perhydrohistrionicotoxin.
The effects of receptor activation were studied on the interaction of perhydrohistrionicotoxin (H(12)-HTX) with the ionic channel of the nicotinic acetylcholine (AcCho) receptor in membranes from the electric organ of Torpedo ocellata and with the endplate region of the soleus muscle of the rat. In Torpedo membranes, the initial rate (i.e., within 30 sec) of [(3)H]H(12)-HTX bindings to the ioni...
متن کاملThe Acetylcholine Receptor Channel from Torpedo
We recorded single channel currents activated by agonist binding to purified acetylcholine receptors from the electric organ of Torpedo californica reconstituted in planar lipid bilayers. Analysis of single channel records indicate that the acetylcholine receptor channel displays two kinetically distinct open states which differ in their mean open times but have similar channel conductances. Th...
متن کاملScanning tunneling microscopy imaging of Torpedo acetylcholine receptor.
The synaptic surface of the acetylcholine receptor in membranes from Torpedo californica electric organ has been imaged by scanning tunneling microscopy. The molecule appears pentameric, with one major and four minor protrusions rising above the surface, and these protrusions encompass a large central cavity. The outer diameter of the molecule is 69 +/- 10 A, while the diameter of the cavity, m...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1979
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.76.6.2576